LUPUS ANTICOAGULANT EVALUATION WITH REFLEX

Handling: 3 ML FROZEN PLATELET-POOR CITRATED PLASMA IN A PLASTIC VIAL INSTRUCTIONS: DRAW BLOOD IN BLUE-TOP TUBE CONTAINING 3.2% SODIUM CITRATE. CENTRIFUGE LIGHT BLUE-TOP TUBE 15 MINUTES AT APPROXIMATELY 1500G WITHIN 60 MINUTES OF COLLECTION. USING A PLASTIC PIPETTE, REMOVE PLASMA, TAKING CARE TO AVOID THE WBC/PLATELET BUFFY LAYER AND PLACE INTO A PLASTIC VIAL. CENTRIFUGE A SECOND TIME AND TRANSFER PLATELET-POOR PLASMA INTO A NEW PLASTIC VIAL. PLASMA MUST BE FREE OF PLATELETS (<10,000/MCL). FREEZE IMMEDIATELY AND SHIP ON DRY ICE., 2 ML, FROZEN